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Scanning Surface Plasmon microscope for surface treatment validation

​We built up a scanning surface plasmon microscope for near-surfaced refractive index image. It has been applied to DNA sensor (nucleotide probe modification), immunoassay (PlGF cancer biomarker), and graphene image (layer identification).

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Raman optical tweezers for investigating physical and chemical response of RBC treat with reactive oxygen species

We Integrated Raman and optical tweezers in one system. This design could provide physical and chemical information of the detecting material at same time. The effect of reactive oxidase species on red blood cell in deformation ability and hemoglobin was demonstrated  in real time observation.

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Tip-enhanced fluorescence for real-time rare DNA isothermal amplification monitoring

Fluorescence signal from a single spot isothermal amplification was enhanced by  plamon phenomenon and lightning effect on metal nano-tip. This technology allowed real-time observation in a DNA amplification. The application in Hepatitis B and C virus DNA amplification have been published in journal and conferences.

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Digital nanoarray sensor for isothermal DNA amplification

By digitized counting the number of fluorescence spot on nanoarray, we can easily quantify the template in isothermal DNA amplification. We have developed a Hepatitis C virus nanobiosensor based on this technology. In this research, Dip-pen nanolithography and microsphere lithography was used to fabricate nanoarray.

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Plasmonic Droplet Sensor 

Mircrofluidics

By integrating  plasmonic sensor and droplet microfluidics, we established a new platform for single cell secretion analysis. This plasmonic sensor could be detected by resonance frequency shift, metal enhanced fluorescence or surface enhanced Raman spectrum.  

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